Mounting Collodion Sections before Staining

The mass staining of paraffin sections before the removal of wax.

The problem of producing large nwmbers of sections for teaching purposes and staining them by haematoxylin and eosin has become acute in.the post-war years, due to the shortage both of trained technicians and of reagents, especially ethyl alcohol. Methods previously used have suffered from the disadvantages of being too long, or wasteful of reagents, and it was with these factors in mind that t...

Improved method for trypsin digestion of Paraplast sections before immunofluorescence staining.

Formalin-fixed, Paraplast-embedded tissue sections mounted on subbed slides were digested with crude trypsin in modified Sorensen phosphate buffer containing sodium fluoride before immunofluorescence staining. This method prevented detachment of sections from slides during processing, thereby permitting immunofluorescence and histological examinations of adjacent serial sections.

Improved resolution by mounting of tissue sections for laser microdissection.

BACKGROUND Laser microbeam microdissection has greatly facilitated the procurement of specific cell populations from tissue sections. However, the fact that a coverslip is not used means that the morphology of the tissue sections is often poor. AIMS To develop a mounting method that greatly improves the morphological quality of tissue sections for laser microbeam microdissection purposes so t...

Non-nuclear staining of thick tissue sections.

Staining of toxoplasma in histological sections.

EXTRACELLULAR toxoplasma, although present in abundance in smears from animals infected experimentally, can only occasionally be found in sections from suspected toxoplasma lesions. In the eye, Wilder (1952a, b) has described organisms morphologically indistinguishable from toxoplasma in cases of granulomatous uveitis, but even in these sections the organisms were not numerous and were found on...